However, since these modular units lack any sensor or detection system to monitor gas-phase O 2, it is not possible to identify any changes to the O 2 concentration in the chamber for the duration of the hypoxia experiment the O 2 level in the chamber is assumed to remain constant for the duration of the 48–72 hr experiment. Manufacturers recommend a second purge with the gas pre-mix 1 hr later to remove any residual trapped air. The chamber is sealed and placed in a laboratory incubator (37☌) to culture the cells under hypoxic conditions. 4 min at 20 L/min), usually with a pre-formulated gas mixture of 1% O 2, 5% CO 2, and 94% N 2. Tumor cell cultures are placed in the chamber and the chamber purged via a pair of ports for a predetermined time-period (e.g. ![]() These semi-spherical polycarbonate chambers of approximately 8 liters in volume cost approximately $700 for a modular chamber and a gas-flow meter. Billups-Rothenburg, Inc., CA, USA StemCell Technologies, Inc., Vancouver, BC, Canada) to recapitulate the tumor hypoxic conditions during in vitro cell-culture studies. Research laboratories routinely use portable modular incubator chambers (e.g. Although it was previously thought that hypoxic regions are primarily located in the tumor “core”, recent investigations indicate that such hypoxic regions are more heterogeneously distributed within the bulk tumor. The studies suggest that an O 2 concentration of 1.3% or below marks the threshold for altered metabolic changes in tumors metabolism remains relatively unaltered at higher O 2 concentrations. These hypoxic regions help support the tumor against chemotherapy, radiation therapy, and alter the tumor's metabolism in such a way to help it invade and metastasize throughout the patient's body. IS187x_102_BLEDK3v1.11_UIv1.Rapidly growing tumors, particularly those that are malignant, contain regions of low O 2 within the tumor mass. Has anyone got any solutions to the issue? I've also tried previous versions, but still they all have 'connect failed' I've updated the MCP2200 drivers to the latest ones from the microchip website, as well as using the latest versions of the CoolTerm and ISupdater. I've tried many times to get the software to connect, many different ways, without success, and it's frustrating to no end. When I attempt to connect to either BLE module using the ISUpdate software that is recommended in the update steps to use as the firmware updater, I'm not able to connect to the module to update the firmware. The data is still able to be sent and received, so that confirms the baud rate is correct. If I use a BM70 module (which is the same hardware as RN4870 but different firmware), i can't enter any CMD mode, or do any sort of reset. In CoolTerm, I'm able to enter the CMD mode for the RN4870 module to set and read settings of the module, so I can check and set the baudrate to the default 115200. I'm able to send and receive data on the CoolTerm terminal, and on the mobile device apps, so that suggests to me that the USART adaptor is working correctly, and so is the BLE module. ![]() I can see the BLE broadcast in the bluetooth lists, and I can connect to it to use the apps.įor the RN4870 module, I'm able to connect to CoolTerm software to test that I'm getting communication between the terminal and a mobile device. To test the BLE connection and data transfer I have mobile devices (iOS and Android) with the recommended apps (SmartData, mBIoT). I have verified many many many times that my connections are correct. I have the 3.3V, GND, reset switch, mode switch, USART connection from the RN4870 BLE module connected as per the microchip connection diagrams ( ). There seems to be no troubleshooting information available, so I hope that someone has run into the same issues and solved it.įor the hardware setup, I'm connecting to a PC via a USB-USART serial adapter board. ![]() I have a BLE module that i'm trying to update the firmware in, but having a lot of problems connecting the recommended update software (ISupdater).
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